Second mutant RRM2 construct was expressed and purified. The NMR studies of role of second cysteine in RRM2 will be performed.
Tags: RRM2
Posts tagged ‘RRM2’
The RRM2 mutant C244S was expressed and purified
Posted: 01.09.2015This RRM2 mutant lacks cysteine that is presumably oxidized when wild-type RRM2 is treated by hydrogen peroxide. Experiment on this protein will help to understand the role of C244.
Tags: RRM2
First protein expressed in newly established laboratory: RRM2
Posted: 20.01.2015
Here is the gel with analysis of differential expression of RRM2 domain from neuropathological protein TDP-43. On the last lane we observed strong band corresponding to molecular weight of about 10 kDa. Further studies of RRM2 including H/D exchange NMR experiments on 15N-labeled samples will be carried out.
Tags: RRM2
RRM2 1H, 13C, 15N chemical shifts
Posted: 11.06.2014On May 2, 2014 in repository Biological Magnetic Resonance Data Bank the assignments of 1H, 13C and 15N chemical shifts for RRM2 domain of TDP-43 protein was published.
Authors: Rabdano, Sevastyan; Podkorytov, Ivan; Yuwen, Tairan; Groves, Adam; Chamberlain, Kyle; Yakimov, Alexander; Skrynnikov, Nikolai.
Tags: BMRB, Podkorytov, Rabdano, RRM2, Skrynnikov