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Posts tagged ‘Rabdano’

The practical course “Cloning strategies”

Posted: 01.12.2015

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Sevastyan Rabdano and Yulia Pivovarova attended two-week course on cloning in Moscow State University organized by Anna Golovina in Olga Dontsova’s lab (A.N. Belozersky Institute of Physico-Chemical Biology MSU). They were trained to perform cloning of DNA constructs to expression vectors including such operations like DNA electrophoresis, DNA extraction, PCR, restriction analysis.

Sevastyan presented the oral talk about projects in Biomolecular NMR laboratory at weekly group seminar.

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Spinus 2015 conference

Posted: 15.11.2015

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Sevastyan have attended the Spinus “Magnetic resonance and its applications” conference in Repino, St. Petersburg. The oral talk entitled “Последствия окислительного стресса для структуры и стабильности РНК-распознающего домена RRM2 белка TDP-43” was presented.

Sevastyan received main prize for best oral talk funded by Uwe and Barabara Eichhoff.

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“Hydration Shells” of CH2 Groups of ω-Amino Acids as Studied by Deuteron NMR Relaxation

Posted: 22.10.2015

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Rabdano, S.O., Donets, A.V., Vovk, M.A., Michel, D., Chizhik, V.I., 2015. “Hydration Shells” of CH 2 Groups of ω-Amino Acids as Studied by Deuteron NMR Relaxation. The Journal of Physical Chemistry B 119, 13358–13366 (2015).
DOI: 10.1021/acs.jpcb.5b06584

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XII International Workshop On Magnetic Resonance, Rostov-on-Don

Posted: 02.03.2015

Sergei Izmailov, Sevastyan Rabdano, Ivan Podkorytov and Nikolai Skrynnikov attended conference “XII International Symposium on Magnetic Resonance (Spectroscopy, Imaging & Ecology)”, Rostov-on-Don, Russia, 2-6 March 2015.

Nikolai Skrynnikov presented lecture “Probing protein dynamics by NMR experiments and MD simulations”.

Sevastyan presented an oral talk “Destabilization of RNA-Recognition Motif RRM2 In Proteinopathic Protein TDP-43 Under the Effect of Oxidative Stress”.

Sergei Izmailov presented a poster entitled “Molecular dynamics simulation of folding of cysteine-containing peptide guanylin with formation of disulfide bonds”

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EMBO course “Multidimensional NMR in Structural Biology”

Posted: 10.08.2014

EMBO course

Sevastyan Rabdano attended the EMBO course Multidimensional NMR in Structural Biology organized by R. Boelens, C. Griesinger and H. Oschkinat in Berlin. Along with 35 selected graduate students he learned the basic and comprehensive techniques in biomolecular NMR. The topics discussed on lectures are listed below:

  • NMR theory (product operator formalism)
  • Pulse sequences involving pulsed field gradients
  • Multidimensional and multinuclear NMR
  • Methods for spectral assignment
  • Partial alignment of biomolecules
  • Relaxation theory and dynamics
  • Structure calculations and computational methods
  • Protein expression and isotope labeling
  • Solid state NMR
  • NMR enhancement methods (hyperpolarization and DNP)

At the poster session Sevastyan presented the results of work on RRM2. The poster title was “RNA‐recognition motif RRM2 in TDP-43: oxidative stress leads to conformational change, proteolysis, formation of inclusion bodies, and neurodegenerative disease”.

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RRM2 1H, 13C, 15N chemical shifts

Posted: 11.06.2014

On May 2, 2014 in repository Biological Magnetic Resonance Data Bank the assignments of 1H, 13C and 15N chemical shifts for RRM2 domain of TDP-43 protein was published.

Authors: Rabdano, Sevastyan; Podkorytov, Ivan; Yuwen, Tairan; Groves, Adam; Chamberlain, Kyle; Yakimov, Alexander; Skrynnikov, Nikolai.

BMRB 19922

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The study of hydration properties of functional groups of glycine and beta-alanine amino acids by nuclear magnetic resonance and quantum chemical calculations

Posted: 10.06.2014

Sevastyan Rabdano have defended master thesis “The study of hydration properties of functional groups of glycine and beta-alanine amino acids by nuclear magnetic resonance and quantum chemical calculations”.

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NMRCM 2014

Posted: 07.06.2014

Members of the laboratory took active part in conference Nuclear Magnetic Resonance in Condenced Matter “Biomolecular NMR and related phenomena”, Saint Petersburg, July 7-11, 2014.

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Nikolai Skrynnikov presented the lecture

  • T. Yuwen, Yi Xue, F. Zhu and N.R. Skrynnikov Modeling a system with intrinsic disorder: an NMR/MD study of peptide-protein encounter complex.

Sevastyan Rabdano presented oral talk

  • The study of hydration properties of functional groups of glycine and beta- alanine amino acids by nuclear magnetic resonance and quantum chemical calculations.

All member of laboratory participated in discussion of lectures, oral and poster presentations at the NMRCM 2014.

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