Olga Rogacheva have defended PhD thesis “Molecular modeling of activation mechanism of protein kinase A Ia”.
The information about thesis is available at the web-site of SPbU.
Tags: Rogacheva
XII International Workshop On Magnetic Resonance, Rostov-on-Don
Posted: 02.03.2015Sergei Izmailov, Sevastyan Rabdano, Ivan Podkorytov and Nikolai Skrynnikov attended conference “XII International Symposium on Magnetic Resonance (Spectroscopy, Imaging & Ecology)”, Rostov-on-Don, Russia, 2-6 March 2015.
Nikolai Skrynnikov presented lecture “Probing protein dynamics by NMR experiments and MD simulations”.
Sevastyan presented an oral talk “Destabilization of RNA-Recognition Motif RRM2 In Proteinopathic Protein TDP-43 Under the Effect of Oxidative Stress”.
Sergei Izmailov presented a poster entitled “Molecular dynamics simulation of folding of cysteine-containing peptide guanylin with formation of disulfide bonds”
Tags: Izmailov, Podkorytov, Rabdano, Skrynnikov
First protein expressed in newly established laboratory: RRM2
Posted: 20.01.2015
Here is the gel with analysis of differential expression of RRM2 domain from neuropathological protein TDP-43. On the last lane we observed strong band corresponding to molecular weight of about 10 kDa. Further studies of RRM2 including H/D exchange NMR experiments on 15N-labeled samples will be carried out.
Tags: RRM2
New postdoc Kerstin Kämpf from Germany
Posted: 16.01.2015
Welcome to our new postdoc Kerstin Kämpf from Germany. Kerstin is going to investigate the dynamics of the disordered tail of histone H4 with NMR and MD simulations.
Tags: Kaempf
The lab setup started
Posted: 25.12.2014Today the wagon with equipment and furniture have arrived to Petergof. We started installation of equipment to our three lab rooms and four office rooms. We have everything now to start expression and purification of proteins. Starting from shaking incubator to FPLC system everything is new and shiny.
Modeling a system with intrinsic disorder: an NMR/MD study of peptide-protein encounter complex
Posted: 24.08.2014
Nikolai Skrynnikov has attended the International Conference on Magnetic Resonance in Biological Systems (ICMRBS), Dallas, TX, 24-29 Aug 2014. He presented the lecture “Modeling a system with intrinsic disorder: an NMR/MD study of peptide-protein encounter complex”.
Tags: Skrynnikov
EMBO course “Multidimensional NMR in Structural Biology”
Posted: 10.08.2014
Sevastyan Rabdano attended the EMBO course Multidimensional NMR in Structural Biology organized by R. Boelens, C. Griesinger and H. Oschkinat in Berlin. Along with 35 selected graduate students he learned the basic and comprehensive techniques in biomolecular NMR. The topics discussed on lectures are listed below:
- NMR theory (product operator formalism)
- Pulse sequences involving pulsed field gradients
- Multidimensional and multinuclear NMR
- Methods for spectral assignment
- Partial alignment of biomolecules
- Relaxation theory and dynamics
- Structure calculations and computational methods
- Protein expression and isotope labeling
- Solid state NMR
- NMR enhancement methods (hyperpolarization and DNP)
At the poster session Sevastyan presented the results of work on RRM2. The poster title was “RNA‐recognition motif RRM2 in TDP-43: oxidative stress leads to conformational change, proteolysis, formation of inclusion bodies, and neurodegenerative disease”.
Tags: Rabdano
RRM2 1H, 13C, 15N chemical shifts
Posted: 11.06.2014On May 2, 2014 in repository Biological Magnetic Resonance Data Bank the assignments of 1H, 13C and 15N chemical shifts for RRM2 domain of TDP-43 protein was published.
Authors: Rabdano, Sevastyan; Podkorytov, Ivan; Yuwen, Tairan; Groves, Adam; Chamberlain, Kyle; Yakimov, Alexander; Skrynnikov, Nikolai.
Tags: BMRB, Podkorytov, Rabdano, RRM2, Skrynnikov
The study of hydration properties of functional groups of glycine and beta-alanine amino acids by nuclear magnetic resonance and quantum chemical calculations
Posted: 10.06.2014Sevastyan Rabdano have defended master thesis “The study of hydration properties of functional groups of glycine and beta-alanine amino acids by nuclear magnetic resonance and quantum chemical calculations”.
Tags: Rabdano