SDS-PAGE gel 14%: recipe for 4 gels
14 % Gel
| Separating gel | Forming gel | |
| Water milliQ | 2.96 mL | 3.2 mL |
| Acrylamide (30%) | 8 mL | 1.6 mL |
| Tris (1 M), pH 8.8 | 6 mL | — |
| Tris (1 M), pH 6.8 | — | 1 mL |
| Ammonium persulfate/PSA/APS (10%) | 80 uL | 56 uL |
| SDS (10%) | 160 uL | 80 uL |
| TEMED/Tetramethylethylene | 16 uL | 12 uL |
Total volume is enough for 4 gels (BIO-RAD Mini-PROTEAN).