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Biophysical Society 60th annual meeting

Posted: 25.02.2016

Biophysical Society 60th annual meeting

Sevastyan Rabdano and Sergei Izmailov attended Biophysical Society 60th annual meeting, Los Angeles, California, USA. They presented results of RRM2 and Guanylin oxidative folding projects.

Sevastyan presented poster “Loss of protein stability due to formation of intermolecular disulfide bonds under the effect of oxidative stress: case study of the RRM2 domain from neuropathological protein TDP-43”.

Sergei presented poster “MD Modeling of Oxidative Folding in Peptides and Proteins”.

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The RRM2 mutant C198S was expressed and purified

Posted: 22.02.2016

Second mutant RRM2 construct was expressed and purified. The NMR studies of role of second cysteine in RRM2 will be performed.

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The practical course “Cloning strategies”

Posted: 01.12.2015

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Sevastyan Rabdano and Yulia Pivovarova attended two-week course on cloning in Moscow State University organized by Anna Golovina in Olga Dontsova’s lab (A.N. Belozersky Institute of Physico-Chemical Biology MSU). They were trained to perform cloning of DNA constructs to expression vectors including such operations like DNA electrophoresis, DNA extraction, PCR, restriction analysis.

Sevastyan presented the oral talk about projects in Biomolecular NMR laboratory at weekly group seminar.

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Spinus 2015 conference

Posted: 15.11.2015

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Sevastyan have attended the Spinus “Magnetic resonance and its applications” conference in Repino, St. Petersburg. The oral talk entitled “Последствия окислительного стресса для структуры и стабильности РНК-распознающего домена RRM2 белка TDP-43” was presented.

Sevastyan received main prize for best oral talk funded by Uwe and Barabara Eichhoff.

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“Hydration Shells” of CH2 Groups of ω-Amino Acids as Studied by Deuteron NMR Relaxation

Posted: 22.10.2015

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Rabdano, S.O., Donets, A.V., Vovk, M.A., Michel, D., Chizhik, V.I., 2015. “Hydration Shells” of CH 2 Groups of ω-Amino Acids as Studied by Deuteron NMR Relaxation. The Journal of Physical Chemistry B 119, 13358–13366 (2015).
DOI: 10.1021/acs.jpcb.5b06584

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15N labeled H4-tail HSQC

Posted: 12.10.2015

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Finally the H4-tail has been successful expressed and now the first HSQC spectrum of this peptide has been recorded. Now the measurements of the dynamics can start!

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The RRM2 mutant C244S was expressed and purified

Posted: 01.09.2015

This RRM2 mutant lacks cysteine that is presumably oxidized when wild-type RRM2 is treated by hydrogen peroxide. Experiment on this protein will help to understand the role of C244.

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Molecular modeling of activation mechanism of protein kinase A Ia

Posted: 21.05.2015

Olga Rogacheva have defended PhD thesis “Molecular modeling of activation mechanism of protein kinase A Ia”.

The information about thesis is available at the web-site of SPbU.

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15N-labeled Mucin

Posted: 16.04.2015

Mucin expression gel

First successful expression of 15N labeled Mucin.

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XII International Workshop On Magnetic Resonance, Rostov-on-Don

Posted: 02.03.2015

Sergei Izmailov, Sevastyan Rabdano, Ivan Podkorytov and Nikolai Skrynnikov attended conference “XII International Symposium on Magnetic Resonance (Spectroscopy, Imaging & Ecology)”, Rostov-on-Don, Russia, 2-6 March 2015.

Nikolai Skrynnikov presented lecture “Probing protein dynamics by NMR experiments and MD simulations”.

Sevastyan presented an oral talk “Destabilization of RNA-Recognition Motif RRM2 In Proteinopathic Protein TDP-43 Under the Effect of Oxidative Stress”.

Sergei Izmailov presented a poster entitled “Molecular dynamics simulation of folding of cysteine-containing peptide guanylin with formation of disulfide bonds”

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